Downloadable Tool

Function

Website

FastPCR

Primer design; determination of the primer length, the melting temperature, the virtual quality of the oligonucleotide, the molecular weight, the minimal and maximal optimal annealing temperature for unknown PCR product, presence of primer-dimer; Sequence manipulation which includes reverse, complement and reverse-complement transformation; DNA-to-protein translation in all the 6 reading frames; and protein-to-DNA translation; cleaning sequences from non-DNA letters; repeats or microsatellite search

http://www.biocenter.helsinki.fi/bi/Programs/ download.htm

BioEdit

Plasmid drawing

http://www.mbio.ncsu.edu/BioEdit/ bioedit.html

CLUSTALX and Phylip programs

Making a phylogenetic tree using

http://www.protein.sdu.dk/bm131/html/downloads.html and

www.evolution.genetics.washington.edu/ phylip/getme.html

MW program

Calculating the molecular weight of a compound

http://publish.uwo.ca/~jkiernan/filelist.htm